Path BioResource welcomes back Penn researchers!

100% operations restored for FY22, effective 7/6/2021.

Please contact PBRHELPDESK@pennmedicine.upenn.edu re: questions or concerns.

NUCLEIC ACID EXTRACTION

DNA Extraction

Automated Extraction from 0.2 ml BUFFY COAT
Automated Extraction from 0.3 ml BLOOD
Automated Extraction from 3.0 ml BLOOD
Automated Extraction from 10.0 ml BLOOD
Automated Extraction from 1.0 ml SERUM
Automated Extraction from 0.6 ml ORAGENE SALIVA
Automated Extraction from 4.0 ml ORAGENE SALIVA
Automated Extraction from 10 mg TISSUE
Manual DNA extraction per sample: other input options

RNA Extraction

from TISSUE
from CELL
from PAXGENE BLOOD
We DO NOT offer RNA extraction service only; it is offered for projects with downstream applications done in our Facility.

GENOTYPING: DETECTION OF KNOWN VARIATIONS

Single Assay: Applied Biosystems

SNP Assay Validation by Taqman using Coriell Controls
SNP Genotyping by Taqman

Multiple Assays: Fluidigm

48.48 Array
96.96 Array

Genome Wide Assay: Affymetrix

Human SNP Array 6.0 (Single Chip)
Axiom on Gene Titan (16, 24 and 96 Format Array Plates)

Genome-wide Chromosomal Analysis aCGH Agilent

2 x 400K, 4 x 180K, 8 x 60 K arrays, standard and FFPE samples

GENE EXPRESSION

Single Assay: Applied Biosystems

cDNA synthesis
Gene Expression Assay by Taqman

Multiple Assays: Fluidigm

48.48 Array
96.96 Array
Preparing STA Product for Sorted Cells (1-100 Cells per Well)
We can do Taqman assays and DELTAgene Assays. We do not do Primer Optimization.

Multiplexed Assays: Luminex

QuantiGene Plex assay (Panomics/Affymetrix)

Microarray: Affymetrix (Affymetrix Catalog)

Exon Arrays
Gene Arrays
Human Transcriptome Arrays
3' Expression Arrays
Gene Titan 96 samples
Gene Titan 24 samples
Gene Titan 16 samples
miRNA Profiling
Affymetrix array hybridization and scan per GeneChip

DIGITAL PCR

Fluidigm

12.765 Digital Array
48.770 DigitalArray

MULTIPLEXED AMPLICON LIBRARY PREPARATION FOR NEXT GENERATION SEQUENCING

GENERAL LAB SERVICES

Bioanalyzer, Full Service (12 samples nano or 11 samples for pico)
Self Service (QuantStudio12K Flex, Bioanalyzer, Fluidigm, Luminex Flexmap 3D,Nanodrop)
Investigator Specific Assays
Consultation

BIOINFORMATICS

Consultation
Data Management
Programming
Dataset Analysis
Data Storage
Software and Analysis Support
Website Hosting
Workstation Use