Molecular Profiling (Genetics)

NUCLEIC ACID EXTRACTION

DNA Extraction

Automated Extraction from 0.2 ml BUFFY COAT
Automated Extraction from 0.3 ml BLOOD
Automated Extraction from 3.0 ml BLOOD
Automated Extraction from 10.0 ml BLOOD
Automated Extraction from 1.0 ml SERUM
Automated Extraction from 0.6 ml ORAGENE SALIVA
Automated Extraction from 4.0 ml ORAGENE SALIVA
Automated Extraction from 10 mg TISSUE
Manual DNA extraction per sample: other input options

RNA Extraction

from TISSUE
from CELL
from PAXGENE BLOOD
We DO NOT offer RNA extraction service only; it is offered for projects with downstream applications done in our Facility.

GENOTYPING: DETECTION OF KNOWN VARIATIONS

Single Assay: Applied Biosystems

SNP Assay Validation by Taqman using Coriell Controls
SNP Genotyping by Taqman

Multiple Assays: Fluidigm

48.48 Array
96.96 Array

Genome Wide Assay: Affymetrix

Human SNP Array 6.0 (Single Chip)
Axiom on Gene Titan (16, 24 and 96 Format Array Plates)

Genome-wide Chromosomal Analysis aCGH Agilent

2 x 400K, 4 x 180K, 8 x 60 K arrays, standard and FFPE samples

GENE EXPRESSION

Single Assay: Applied Biosystems

cDNA synthesis
Gene Expression Assay by Taqman

Multiple Assays: Fluidigm

48.48 Array
96.96 Array
Preparing STA Product for Sorted Cells (1-100 Cells per Well)
We can do Taqman assays and DELTAgene Assays. We do not do Primer Optimization.

Multiplexed Assays: Luminex

QuantiGene Plex assay (Panomics/Affymetrix)

Microarray: Affymetrix (Affymetrix Catalog)

Exon Arrays
Gene Arrays
Human Transcriptome Arrays
3' Expression Arrays
Gene Titan 96 samples
Gene Titan 24 samples
Gene Titan 16 samples
miRNA Profiling
Affymetrix array hybridization and scan per GeneChip

DIGITAL PCR

Fluidigm

12.765 Digital Array
48.770 DigitalArray

MULTIPLEXED AMPLICON LIBRARY PREPARATION FOR NEXT GENERATION SEQUENCING

Fluidigm

Access Array System

GENERAL LAB SERVICES

Bioanalyzer, Full Service (12 samples nano or 11 samples for pico)
Self Service (QuantStudio12K Flex, Bioanalyzer, Fluidigm, Luminex Flexmap 3D,Nanodrop)
Investigator Specific Assays
Consultation

BIOINFORMATICS

Consultation
Data Management
Programming
Dataset Analysis
Data Storage
Software and Analysis Support
Website Hosting
Workstation Use


	Molecular Profiling (Genetics)
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	NUCLEIC ACID EXTRACTION DNA Extraction Automated Extraction from 0.2 ml BUFFY COAT Automated Extraction from 0.3 ml BLOOD Automated Extraction from 3.0 ml BLOOD Automated Extraction from 10.0 ml BLOOD Automated Extraction from 1.0 ml SERUM Automated Extraction from 0.6 ml ORAGENE SALIVA Automated Extraction from 4.0 ml ORAGENE SALIVA Automated Extraction from 10 mg TISSUE Manual DNA extraction per sample: other input options RNA Extraction from TISSUE from CELL from PAXGENE BLOOD We DO NOT offer RNA extraction service only; it is offered for projects with downstream applications done in our Facility. GENOTYPING: DETECTION OF KNOWN VARIATIONS Single Assay: Applied Biosystems SNP Assay Validation by Taqman using Coriell Controls SNP Genotyping by Taqman Multiple Assays: Fluidigm 48.48 Array 96.96 Array Genome Wide Assay: Affymetrix Human SNP Array 6.0 (Single Chip) Axiom on Gene Titan (16, 24 and 96 Format Array Plates) Genome-wide Chromosomal Analysis aCGH Agilent 2 x 400K, 4 x 180K, 8 x 60 K arrays, standard and FFPE samples GENE EXPRESSION Single Assay: Applied Biosystems cDNA synthesis Gene Expression Assay by Taqman Multiple Assays: Fluidigm 48.48 Array 96.96 Array Preparing STA Product for Sorted Cells (1-100 Cells per Well) We can do Taqman assays and DELTAgene Assays. We do not do Primer Optimization. Multiplexed Assays: Luminex QuantiGene Plex assay (Panomics/Affymetrix) Microarray: Affymetrix (Affymetrix Catalog) Exon Arrays Gene Arrays Human Transcriptome Arrays 3' Expression Arrays Gene Titan 96 samples Gene Titan 24 samples Gene Titan 16 samples miRNA Profiling Affymetrix array hybridization and scan per GeneChip DIGITAL PCR Fluidigm 12.765 Digital Array 48.770 DigitalArray MULTIPLEXED AMPLICON LIBRARY PREPARATION FOR NEXT GENERATION SEQUENCING Fluidigm Access Array System GENERAL LAB SERVICES Bioanalyzer, Full Service (12 samples nano or 11 samples for pico) Self Service (QuantStudio12K Flex, Bioanalyzer, Fluidigm, Luminex Flexmap 3D,Nanodrop) Investigator Specific Assays Consultation BIOINFORMATICS Consultation Data Management Programming Dataset Analysis Data Storage Software and Analysis Support Website Hosting Workstation Use