Path BioResource welcomes back Penn researchers!

100% operations restored for FY22, effective 7/6/2021.

Please contact PBRHELPDESK@pennmedicine.upenn.edu re: questions or concerns.

The Facility operates on a cost recovery basis and all fees will reflect the actual expenses of reagents, consumable labware and service but not the instrument purchase.

Projects for clients not affiliated with the University of Pennsylvania, CHOP or Wistar will be assessed a surcharge on all Facility fees.

The Facility will NOT be responsible for data after 6 MONTHS from the date of report.

The Facility will NOT be responsible for any samples/reagents after 1 MONTH from the date of report.

Please collect samples and related assays/reagents and download the data from our server as soon as we report.

NUCLEIC ACID EXTRACTION
DNA Extraction: Per SampleCost
Automated 0.2 ml BUFFY COAT per sample, avg yield 5 ug (BATCH OF 96)$15.00
Automated DNA from 0.3 ml BLOOD per sample, avg yield 8 ug (BATCH OF 96)$15.00
Automated DNA from 3.0 ml BLOOD per sample, avg yield 60 ug (BATCH OF 12)$22.00
Automated DNA from 10.0 ml BLOOD per sample, avg yield 200 ug (BATCH OF 12)$35.00
Automated DNA from 1.0 ml SERUM per sample, avg yield 30 ng (BATCH OF 96)$15.00
Automated DNA from 0.6 ml ORAGENE SALIVA per sample, avg yield 15 ug (BATCH OF 96)$15.00
Automated DNA from 4.0 ml ORAGENE SALIVA per sample, avg yield 50 ug (BATCH OF 12)$22.00
Automated DNA from 10 mg TISSUE per sample, average yield varies with type of tissue (BATCH OF 96)$15.00
Manual DNA extraction per sample, other input options$50.00

NOTE:

    If NOT submitted in full batch size, additional labor charge will be applied for one batch to recover cost of the plastics.
    The Samples must be submitted in the same order as listed in the Sample sheet.

RNA Extraction: Per Sample
RNA Extraction from TISSUE, per sample$50.00
RNA Extraction from CELL, per sample$50.00
RNA Extraction from PAXGENE BLOOD, per sample$50.00

NOTE:

    We DO NOT offer RNA extraction service only; it is offered for projects with downstream applications done in our Facility.
    The Samples must be submitted in the same order as listed in the Sample sheet.

GENOTYPING: DETECTION OF KNOWN VARIATIONS
Single Assay: Applied Biosystems
SNP Assay Validation by Taqman using Coriell Controls$75.00
SNP Genotyping; 1 SNP, per genotype by Taqman$1.10
SNP Genotyping; 2 SNPs, per genotype by Taqman$0.80
SNP Genotyping; 3 SNPs, per genotype by Taqman$0.70
SNP Genotyping; 4 SNPs, per genotype by Taqman$0.65
SNP Genotyping; 5-9 SNPs, per genotype by Taqman0.60
SNP Genotyping; 10 or more SNPs, per genotype by Taqman0.55
SNP Genotyping; 30 or more SNPs, per genotype by Taqman0.50
QuantStudio 12K Flex, Self Service$120.00

NOTE:

    Additional labor charges will be applied if samples are NOT submitted following our Sample Submision Protocol or submitted less than a batch of 384. The samples must be submitted in the same order as listed in the Sample or Assay sheet.

Multiple Assays: Fluidigm
48.48 GT Array$900.00
96.96 GT Array$1,400.00
Preparing STA Product for 48:48 Array$350.00
Preparing STA Product for 96:96 Array$400.00

NOTE:

    Additional labor charges will be applied if samples are NOT submitted following our Sample Submision Protocol or submitted less than a batch of 48 or 96 as applies. The Samples and Assays must be submitted in the same order as listed in the Sample or Assay sheet.

Genome Wide Assay: Affymetrix (Batch Size:12)
DNA QC (up to 96 samples)$50.00
Human SNP Array 6.0$700.00

NOTE:

    Additional labor charges will be applied if samples are NOT submitted following our Sample Submision Protocol.

Genome-wide Chromosomal Analysis aCGH Agilent
2 X 400K array (2 samples/slide), per sample$360.00
2 X 400K array (2 samples/slide), per FFPE sample$325.00
4 X 180K array (4 samples/slide), per sample$240.00
4 X 180K array (4 samples/slide), per FFPE sample$185.00
8 X 60K array (8 samples/slide), per FFPE sample$130.00

GENE EXPRESSION
Single Assay: Applied Biosystems
cDNA synthesis per RNA sample$6.00
Gene Expression Assay Set-up, per 384 well assay plate used$180.00
Gene Expression Assay by Taqman, per sample in quadruplicate$5.00
QuantStudio 12K Flex, Self Service$120.00

NOTE:

    Additional labor charges will be applied if samples are NOT submitted following our Sample Submision Protocol or submitted less than a batch of 96. The Samples and Assays must be submitted in the same order as listed in the Sample or Assay sheet.

Multiple Assays: Fluidigm
48.48 GE Array$1,400.00
96.96 GE Array$2,000.00
Preparing STA Product for single cell (per plate)$180.00
Self Service$180.00

NOTE:

    Single Cell Submission: Please contact the Facility.

    Additional labor charges will be applied if samples are NOT submitted following our Sample Submision Protocol or submitted less than a batch of 48 or 96 as applies. The samples must be submitted in the same order as listed in the Sample or Assay sheet.

Multiplexed Assays: Luminex
QuantiGene Plex Assay (Panomics/ Affymetrix)By Quote

NOTE:

    Additional labor charges will be applied if samples are NOT submitted following our Sample Submision Protocol or submitted less than a batch of 96. The Samples and Assays must be submitted in the same order as listed in the Sample or Assay sheet.

Microarray: Affymetrix
3' IVT Expression Analysis
Human Genome U133 plus 2.0 Array$590.00
Mouse Genome 430 2.0 Array$590.00
Rat Genome 230 2.0 Array$590.00
Other species CHIPS ONLYBy Quote
Other species REAGENTS ONLY$125.00
miRNA Analysis
miRNA 4.0 array$340.00
Whole Transcriptome Expression Analysis Profiling
Human Transcriptome 2.0 array (WT Plus)$510.00
Human Transcriptome 2.0 array (WT Pico)$535.00
Mouse Transcriptome 2.0 array (WT Plus)$510.00
Mouse Transcriptome 2.0 array (WT Pico)$535.00
Rat Transcriptome 2.0 array (WT Plus)$510.00
Rat Transcriptome 2.0 array (WT Pico)$535.00
Human Exon 1.0 ST Array (WT Plus)$610 .00
Human Exon 1.0 ST Array (WT Pico)$635.00
Mouse Exon 1.0 ST Array (WT Plus)$610 .00
Mouse Exon 1.0 ST Array (WT Pico)$635.00
Rat Exon 1.0 ST Array (WT Plus)$610 .00
Rat Exon 1.0 ST Array (WT Pico)$635.00
Human Gene 2.0 ST Array (WT Plus)$500.00
Human Gene 2.0 ST Array (WT Pico)$525.00
Mouse Gene 2.0 ST Array (WT Plus)$500.00
Mouse Gene 2.0 ST Array (WT Pico)$525.00
Rat Gene 2.0 ST Array (WT Plus)$500.00
Rat Gene 2.0 ST Array (WT Pico)$525.00
Model and Applied Research Organism Gene 1.0ST (WT Ambion) CHIPS ONLYBy Quote
Model and Applied Research Organism Gene 1.0ST REAGENTS ONLY$125.00
Model and Applied Research Organism Gene 1.0ST (Pico v2) CHIPS ONLYBy Quote
Model and Applied Research Organism Gene 1.0ST (Pico v2) REAGENTS ONLY$175.00
Affymetrix array hybridization and scan per GeneChip$45.00

NOTE:

    For 3' expression price varies depending on the type of array, view AFFYMETRIX CATALOG. If the samples does not pass QC, we will contact the investigator for RNA resubmission, a charge of $90.00 will be additional for 1-12 samples. If the QC fails again, we will not proceed any further and will be billed for the QC part only.

    If the investigator still wants to proceed with the assay we need documentation for our record. If the result is unsatisfactory, we will bill the full amount. Additional labor charges will be applied if samples are NOT submitted following our Sample Submision Protocol. The Samples must be submitted in the same order as listed in the Sample sheet.

GENERAL LAB SERVICES
SELF SERVICES
InstrumentMinimum Booking Contact InfoCharge per Booking
QuantStudio 12K Flex4 hoursHetty Rodriguez$120.00
Agilent 2100 Bioanalyzer1 hourHetty Rodriguez$35.00
Fluidigm Loader & BioMark HD7 hoursHetty Rodriguez$180.00
Luminex Flexmap 3D7 hoursHetty Rodriguez$200.00
Nanodrop0.5 hourHetty Rodriguez$10.00
OTHER SERVICES
Bioanalyzer, Full Service (12 samples nano or 11 samples for pico)$90.00
Investigator Specific AssaysBy Quote
Additional Labor, per hour$90.00

NOTE:

    Bioanalyzer Self Service charge is just for using the instrument, the facility does not provide chip or reagent.

    Bioanlayzer Full Service is for microarray projects only, if the samples do not pass QC for downstream application we charge for QC only.

BIOINFORMATICS
1TB External Hard Drive$125.00
2TB External Hard Drive$275.00
Bioinformatics consultation per hour$120.00
Bioinformatics data management per hour$120.00
Bioinformatics programming per hour$120.00
Dataset analysis - client session$240.00
Data storage (PGFI SAN) per GB per year$1.20
Data analysis per hour$120.00
GeneSpring subscription per year$1,200.00
Ingenuity subscription per year$2,845.00
Partek subscription per year$1,500.00
Partek static license per year$2,000.00
Sequence analysis software subscription (First 2)$350.00
Sequence analysis software subscription (Each additional)$75.00
Sequence analysis software subscription fiscal year proratedBy Quote
Software and analysis support per hour$120.00
Workstation use (desktop) per hour$25.00
Workstation use (laptop) per hour$25.00
Workstation use (MAC) per hour$25.00
Workstation use (PC) per hour$25.00

Please contact Dr. Tapan Ganguly at gangulyt@pennmedicine.upenn.edu to discuss experimental design, available resources and data analysis.