Dear Molecular Profiling Facility Users,

Effective Jan 2, 2019, the Molecular Profiling Facility will be transitioning to iLab, following in the footsteps of several other PSOM cores. The iLab system is integrated with PSOM’s Central Account Management Services (CAMS).

Our iLab entry portal will be - https://med-upenn.corefacilities.org/service_center/show_external/4515

The new site will be used for:

• All laboratory or bioinformatics service requests
• Self-use equipment reservations
• General information about the core

User Guides with detailed instructions for using our core through iLab are linked below:

Guide for Penn investigators

https://www.bioinformatics.upenn.edu/dl/iLab_user_guide_MPF.pptx

Guide for external investigators (CHOP, Wistar, Monell, off-campus)

https://www.bioinformatics.upenn.edu/dl/iLab_external_user_guide_MPF.pptx

This site will remain live in January for a smooth transition.

The Molecular Profiling Facility provides full service whole genome and targeted molecular profiling of DNA and RNA on multiple platforms. The Facility offers a range of cost and performance options suitable for a variety of experimental projects. While the Facility staff performs most of the assays, investigator-performed studies are actively encouraged through the sharing of Facility procedures, individualized training of investigators or their technical staff, and use of core equipment.

The Core supports quantitative RNA profiling on Affymetrix GeneChips and high-throughput Gene Titan instruments; Luminex FlexMap 3D; Fluidigm BioMark HD; ABI QS 12K real-time PCR machine.

DNA profiling is offered on Affymetrix SNP GeneChip and high-throughput Gene Titan instruments; Fluidigm BioMark HD; ABI QS 12K.

Agilent aCGH platform provides genome-wide chromosomal analysis.

The users benefit from consultation and training available throughout their projects, including during experimental design and budget development, grant preparation, sample accrual, data management and analyses, and manuscript preparation.

For further information, please contact:

Director: Tapan Ganguly, Ph.D. (215-573-7238) gangulyt@pennmedicine.upenn.edu

Technical Director: John Tobias, Ph.D. (215-573-4791) jtobias@pennmedicine.upenn.edu for bioinformatics & experimental design

Hetty Rodriguez, M.S. (215-573-6316) hettymr@pennmedicine.upenn.edu for genotyping & gene expression