HIC opens Monday to Friday from 9am-5pm, except for University defined holidays.

To schedule sample processing: Please email Dr. Ling Zhao (lingzh@pennmedicine.upenn.edu) weekly sample schedule on or before Friday of the preceding week (please include Study PI, UPCC#, sample ID, blood draw/tissue biopsy date and approximate time that samples will be dropped off). Samples received outside of our posted cut-off times require approval at least 24 hours in advance of receipt and additional charges will apply as detailed below.

  1. Same-day processing cut off time: 1:00 PM Monday-Friday. In order to schedule lab staff, samples that need to be processed on the same day that are received after our cut-off time of 1 PM must be pre-approved by Ling Zhao. These samples will incur an after-hours processing fee.
  2. Next-day processing cut off time: 5:00 PM Monday-Thursday. Samples received after 1 PM, but by or before 5 PM, will be processed on the next business day.
  3. Samples received on Friday between 1-5 PM: We offer next-day processing on samples received on Friday between 1 PM and 5 PM, if pre-approved by Ling Zhao. Processing of these samples will incur an after-hours processing fee.
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Academic Publications

Some publications that feature the HIC include:
  1. Schutsky K, Song DG, Lynn R, Smith JB, Poussin M, Figini M, Zhao Y, Powell DJ Jr Rigorous optimization and validation of potent RNA CAR T cell therapy for the treatment of common epithelial cancers expressing folate receptor. 2015 Oct 6;6(30):28911-28. doi: 10.18632/oncotarget.5029. PMID: 26359629
  2. Panjwani MK, Smith JB, Schutsky K, Gnanandarajah J, O’Connor CM, Powell DJ Jr, Mason NJ. Feasibility and Safety of RNA-transfected CD20-specific Chimeric Antigen Receptor T Cells in Dogs with Spontaneous B Cell Lymphoma. Mol Ther. 2016 Sep;24(9):1602-14. doi: 10.1038/mt.2016.146. Epub 2016 Jul 12. PMID: 27401141
  3. Liu XBarrett DMJiang SFang CKalos MGrupp SAJune CHZhao Y Improved anti-leukemia activities of adoptively transferred T cells expressing bispecific T-cell engager in mice. Blood Cancer J.2016 Jun 3;6(6):e430. doi: 10.1038/bcj.2016.38.
  4. Lynn RC1, Feng Y2, Schutsky K1, Poussin M1, Kalota A3, Dimitrov DS2, Powell DJ Jr1,4. High-affinity FRβ-specific CAR T cells eradicate AML and normal myeloid lineage without HSC toxicity. 2016 Jun;30(6):1355-64. doi: 10.1038/leu.2016.35. Epub 2016 Feb 22. PMID: 26898190
  5. Bartman CR, Hsu SC, Hsiung CC, Raj A, Blobel GA Enhancer Regulation of Transcriptional Bursting Parameters Revealed by Forced Chromatin Looping. Mol Cell. 2016 Apr 21;62(2):237-47. doi: 10.1016/j.molcel.2016.03.007. Epub 2016 Apr 7 PMID: 27067601
  6. Liu XRanganathan RJiang SFang CSun JKim SNewick KLo AJune CHZhao YMoon EK A Chimeric Switch-Receptor Targeting PD1 Augments the Efficacy of Second-Generation CAR T Cells in Advanced Solid Tumors. Cancer Res.2016 Mar 15;76(6):1578-90. doi: 10.1158/0008-5472.CAN-15-2524.
  7. Beata Derfalvi , Kelly Maurer , Donna M. McDonald McGinn , Elaine Zackai , Wenzhao Meng , Eline T. Luning Prak , Kathleen E. Sullivan. “B cell development in chromosome 22q11.2 deletion syndrome.” Immunol. 2016, 163: 1-9. PMID: 26689329
  8. Martinez NM, Agosto L, Qiu J, Mallory MJ, Gazzara MR, Barash Y, Fu XD, Lynch KW. Widespread JNK-dependent alternative splicing induces a positive feedback loop through CELF2-mediated regulation of MKK7 during T-cell activation. Genes Dev. 2015 Oct 1;29(19):2054-66. doi: 10.1101/gad.267245.115. PMID: 26443849
  9. Liu XJiang SFang CYang SOlalere DPequignot ECCogdill APLi NRamones MGranda BZhou LLoew AYoung RMJune CHZhao Y. Affinity-Tuned ErbB2 or EGFR Chimeric Antigen Receptor T Cells Exhibit an Increased Therapeutic Index against Tumors in Mice. Cancer Res.2015 Sep 1;75(17):3596-607. doi: 10.1158/0008-5472.CAN-15-0159.
  10. Zhang, B., Meng, W., Luning Prak, E.T. and U. Hershberg. “Discrimination of germline V genes at different sequencing lengths and mutational burdens: a new tool for identifying and evaluating the reliability of V gene assignment.”  Immunol. Methods. Pii: S00022-1759, 2015.  PMID: 26529062.
  11. Mallory MJ, Allon SJ, Qiu J, Gazzara MR, Tapescu I, Martinez NM, Fu XD, Lynch KW. Induced transcription and stability of CELF2 mRNA drives widespread alternative splicing during T-cell signaling. Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):E2139-48. doi: 10.1073/pnas.1423695112. Epub 2015 Apr 13. PMID: 25870297
  12. Frigault MJLee JBasil MCCarpenito CMotohashi SScholler JKawalekar OUGuedan SMcGettigan SEPosey AD JrAng SCooper LJPlatt JMJohnson FBPaulos CMZhao YKalos MMilone MCJune CH. Identification of chimeric antigen receptors that mediate constitutive or inducible proliferation of T cells. Cancer Immunol Res.2015 Apr;3(4):356-67. doi: 10.1158/2326-6066.CIR-14-0186. Epub 2015 Jan 19.
  13. Trimble, C.L., Morrow, M.P., Kraynyak, K.A., Shen, X., Dallas, M., Edwards, L., Parker, R.L., Denny, L., Giffear, M., Brown, A.S., Marcozzi-Pierce, K., Shah, D., Slager, A.M., Sylvester, A.J., Khan, A., Broderick, K.E., Juba, R.J., Herring, T.A., Boyer, J., Lee, J., Sardesai, N.Y., Weiner, D.B. and M.L. Bagarazzi. “Safety, efficacy, and immunogenicity of VGX-3100, a therapeutic synthetic DNA vaccine targeting human papillomavirus 16 and 18 E6 and E7 proteins for cervical intraepithelial neoplasia 2/3: a randomized, double-blind, placebo-controlled phase 2b trial.”    2015. pii: S0140-6737. PMID: 26386540.
  14. Bauml, J., Chen, L., Chen, J., Boyer, J., Kalos, M., Li, S.Q., DeMichele, A. and J.J. Mao, Arthralgia among women taking aromatase inhibitors: is there a shared inflammatory mechanism with co-morbid fatigue and insomnia? Breast Cancer Res.  2015, 17:89. PMID: 26126656.
  15. Morrow, M.P., Tebas, P., Yan, J., Ramirez, L., Slager, A., Kraynyak, K., Diehl, M., Shah, D., Khan, A., Lee, J., Boyer, J., Kim, J.J., Sardesai, N.Y., Weiner, D.B. and M.L. Bagarazzi. “Synthetic consensus HIV-1 DNA induces potent cellular immune responses and synthesis of granzyme B, perforin in HIV-infected individuals.”  Ther.  2015 Vol. 23(3): 591-601.  PMID: 25531694.
  16. Shin J, Maekawa T, Abe T, Hajishengallis E, Hosur K, Pyaram K, Mitroulis I, Chavakis T, Hajishengallis G. DEL-1 restrains osteoclastogenesis and inhibits inflammatory bone loss in nonhuman primates. Sci Transl Med. 2015 Sep 30;7(307):307ra155. doi: 10.1126/scitranslmed.aac5380. PMID: 26424570
  17. Ravimohan S, Tamuhla N, Kung SJ, Nfanyana K, Steenhoff AP, Gross R, Weissman D, Bisson GP. Matrix Metalloproteinases in Tuberculosis-Immune Reconstitution Inflammatory Syndrome and Impaired Lung Function Among Advanced HIV/TB Co-infected Patients Initiating Antiretroviral Therapy. 2015 Nov 25;3:100-7. doi: 10.1016/j.ebiom.2015.11.040. eCollection 2016. PMID: 27014741
  18. Ravimohan S, Tamuhla N, Steenhoff AP, Letlhogile R, Nfanyana K, Bellamy SL, MacGregor RR, Gross R, Weissman D, Bisson GP. Immunological profiling of tuberculosis-associated immune reconstitution inflammatory syndrome and non-immune reconstitution inflammatory syndrome death in HIV-infected adults with pulmonary tuberculosis starting antiretroviral therapy: a prospective observational cohort study. Lancet Infect Dis. 2015 Apr;15(4):429-38. doi: 10.1016/S1473-3099(15)70008-3. Epub 2015 Feb 9. PMID: 25672566
  19. Abe T1, Shin J1, Hosur K1, Udey MC2, Chavakis T3, Hajishengallis G Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8. J Immunol. 2014 Aug 1;193(3):1383-91. doi: 10.4049/jimmunol.1400970. PMID: 24958900
  20. Maldonado L, Teague JE, Morrow MP, Jotova I, Wu TC, Wang C, Desmarais C, Boyer JD, Tycko B, Robins HS, Clark RA and Trimble CL, Intramuscular Therapeutic Vaccination Targeting HPV16 Induces T Cell Responses That Localize in Mucosal Lesions, Science Translational Med, 221, 2014. PMID: 24477000.
  21. Singh NLiu XHulitt JJiang SJune CHGrupp SABarrett DMZhao Y. Nature of tumor control by permanently and transiently modified GD2 chimeric antigen receptor T cells in xenograft models of neuroblastoma. Cancer Immunol Res.2014 Nov;2(11):1059-70. doi: 10.1158/2326-6066.CIR-14-0051. Epub 2014 Aug 7.
  22. Shankarling G, Cole BS, Mallory MJ, Lynch KW. Transcriptome-wide RNA interaction profiling reveals physical and functional targets of hnRNP L in human T cells. Mol Cell Biol. 2014 Jan;34(1):71-83. doi: 10.1128/MCB.00740-13. Epub 2013 Oct 28. PMID: 24164894
  23. Barrett DMSingh NLiu XJiang SJune CHGrupp SAZhao Y. Relation of clinical culture method to T-cell memory status and efficacy in xenograft models of adoptive immunotherapy. 2014 May;16(5):619-30. doi: 10.1016/j.jcyt.2013.10.013. Epub 2014 Jan 16.
  24. Zhang Q, Wang HY, Wei F, Liu X, Paterson JC, Roy D, Mihova D, Woetmann A, Ptasznik A, Odum N, Schuster SJ, Marafioti T, Riley JL, Wasik MA Cutaneous T cell lymphoma expresses immunosuppressive CD80 (B7-1) cell surface protein in a STAT5-dependent manner J Immunol. 2014, 192:2913-9. PMID: 24523507.
  25. Zhang Q, Wei F, Wang HY, Liu X, Roy D, Xiong QB, Jiang S, Medvec A, Danet-Desnoyers G, Watt C, Tomczak E, Kalos M, Riley JL, Wasik MA The potent oncogene NPM-ALK mediates malignant transformation of normal human CD4(+) T lymphocytes. Am J Pathol. 2013, 183:1971-80, PMID: 24404580.
  26. Cameron BJ, Gerry AB, Dukes J, Harper JV, Kannan V, Bianchi FC, Grand F, Brewer JE, Gupta M, Plesa G, Bossi G, Vuidepot A, Powlesland AS, Legg A, Adams KJ, Bennett AD, Pumphrey NJ, Williams DD, Binder-Scholl G, Kulikovskaya I, Levine BL, Riley JL, Varela-Rohena A, Stadtmauer EA, Rapoport AP, Linette GP, June CH, Hassan NJ, Kalos M, Jakobsen BK, Identification of a Titin-derived HLA-A1-presented peptide as a cross-reactive target for engineered MAGE A3-directed T cells, Sci Transl Med. 2013,197:1126, PMID: 23926201.
  27. Tebas P, Stein D, Binder-Scholl G, Mukherjee R, Brady T, Rebello T, Humeau L, Kalos M, Papasavvas E, Montaner LJ, Schullery D, Shaheen F, Brennan AL, Zheng Z, Cotte J, Slepushkin V, Veloso E, Mackley A, Hwang WT, Aberra F, Zhan J, Boyer J, Collman RG, Bushman FD, Levine BL, June CH. Antiviral effects of autologous CD4 T cells genetically modified with a conditionally replicating lentiviral vector expressing long anti-sense to HIV. Blood. 2013:121:1524-1533. PMCID: PMC3587318
  28. Clair, E.W., Levesque, M.C., Luning Prak, E.T., Vivino, F.B., Alappat, C.J., Spychala, M.E., Wedgwood, J., McNamara, H., Moser, Sivilis, K.L., Fisher, L. and P. Cohen. “Rituximab therapy for primary Sjogren’s Syndrome: an open-label clinical trial and mechanistic analysis.”  Arthritis Rheum.  Vol. 65(4): 1097-106, 2013.  PMID: 23334994.
  29. Kalams SA, Parker SD, Elizaga M, Metch B, Edupuganti S, Hural J, De Rosa S, Carter DK, Rybczyk K, Frank I, Fuchs J, Koblin B, Kim DH, Joseph P, Keefer MC, Baden LR, Eldridge J, Boyer JD, Sherwat A, Cardinali M, Allen M, Pensiero M, Butler C, Khan AS, Yan JY, Sardesai NY, Kublin JG, and Weiner DB, Safety and Comparative Immunogenicity of an HIV-1 DNA Vaccine in Combination with Plasmid Interleukin 12 and Impact of Intramuscular Electroporation for Delivery, JID, 2013.
  30. Bedoya F, Cheng GS, Leibow A, Zakhary N, Weissler K, Garcia V, Aitken M, Kropf E, Garlick DS, Wherry EJ, Erikson J, Caton AJ. J Viral antigen induces differentiation of Foxp3+ natural regulatory T cells in influenza virus-infected mice, Journal of Immunology 2013 Jun 15; 190(12):6115-25
  31. Ralph Pietrofesa, Jason Turowski, Sonia Tyagi, Floyd Dukes, Evguenia Arguiri, Theresa M Busch, Shannon M Gallagher-Colombo, Charalambos C Solomides, Keith A Cengel and Melpo Christofidou-Solomidou, Radiation mitigating properties of the lignin component in flaxseed BMC Cancer 2013, 13:179
  32. Grupp SA, Luning Prak E, BoyerJD, et al Adoptive Transfer of Autologous T Cells Improves T-cell Repertoire Diversity and Long-term B-cell Function in Pediatric Patients with Neuroblastoma, Clinical Cancer Research, 18:6732–41, 2012.
  33. Bagarazzi ML, Yan J, Morrow MP, Shen X, Parker RL, Lee JC, Giffear M, Pankhong P, Khan AS, Broderick KE, Knott C, Lin F, Boyer JD, Draghia-Akli R,1 C. Jo White CJ,1 J. Joseph Kim JJ, Weiner DB, Sardesai NY, Immunotherapy Against HPV16/18 Generates Potent TH1 and Cytotoxic Cellular Immune Responses, Science Translational Med, 4:155-168, 2012. PMID:23052295.
  34. Rech AJ, Mick R, Martin S, Recio A, Aqui NA, Powell DJ Jr, Colligon TA, Trosko JA, Leinbach LI, Pletcher CH, Tweed CK, DeMichele A, Fox KR, Domchek SM, Riley JL, Vonderheide RH CD25 blockade depletes and selectively reprograms regulatory T cells in concert with immunotherapy in cancer patients, Sci Transl Med. 2012,134, PMID: 22593175
  35. Jawad AF, Luning Prak E, Boyer JD, McDonald-McGinn DM, Zackai E, McDonald K, Sullivan KE A Prospective Study of Influenza Vaccination and a Comparison of Immunologic Parameters in Children and Adults with Chromosome 22q11.2 Deletion Syndrome (DiGeorge Syndrome/Velocardiofacial Syndrome), JCI, 31:927-935, 2011. PMID:21863400
  36. Melpo Christofidou-Solomidou, Sonia Tyagi, Kay-See Tan, Sarah Hagan, Ralph Pietrofesa, Floyd Dukes, Evguenia Arguiri, Daniel F Heitjan, Charalambos C Solomides and Keith A Cengel, Dietary flaxseed administered post thoracic radiation treatment improves survival and mitigates radiation-induced pneumonopathy in mice, BMC Cancer 2011, 11:269.
  37. Akimova TGe GGolovina TMikheeva TWang LRiley JLHancock WW. Histone/protein deacetylase inhibitors increase suppressive functions of human FOXP3+ Tregs, Clin Immunol.2010 Sep;136(3):348-63. doi: 10.1016/j.clim.2010.04.018.
  38. Akimova T1, Beier UHWang LLevine MHHancock WW Helios expression is a marker of T cell activation and proliferation. PLoS One. 2011;6(8):e24226. doi: 10.1371/journal.pone.0024226. Epub 2011 Aug 30. PMID: 21918685
  39. PLoSOne. 2011;6(8):e24226.doi10.1371/journal.pone.0024226.
  40. Stadtmauer EA, Vogl DT, Luning Prak E, Boyer J, Aqui NA, Rapoport AP, McDonald KR, Hou X, Murphy H, Bhagat R, Mangan PA, Chew A, Veloso EA, Levine BL, Vonderheide RH, Jawad AF, June CJ, and Sullivan KE. Transfer of influenza vaccine-primed co-stimulated autologous T cells after stem cell transplantation for multiple myeloma leads to reconstitution of influenza immunity: results of randomized clinical trial, Blood, 2010. PMID:20864577