Services

jorgelab1

KO MICE

Generation of knock-out (KO) mice usually requires the design of the targeting strategy, production of Cas9 mRNA and 2 or more gRNAs, microinjection of one-cell embryos, and screening of founder mice. Double, triple or quadruple KO mice of different genes can be accomplished with one injection.

Please see fees for pricing information.

KI MICE – SMALL SEQUENCES

Generation of knock-in (KI) mice containing small sequences (e.g. point mutations, tags) usually requires the design of the targeting strategy, production of Cas9 mRNA and a gRNA, preparation of donor ssDNA templates, microinjection of one-cell embryos, and screening of founder mice.

Please see fees for pricing information.

CKO MICE

Generation of conditional knock-out (cKO) mice usually requires the design of the targeting strategy, production of Cas9 mRNA and 2 gRNAs, preparation of 2 donor ssDNA templates containing loxp sites, microinjection of one-cell embryos, and screening of founder mice.

Please see fees for pricing information.

KI MICE – LARGE SEQUENCES

Generation of knock-in (KI) mice containing large sequences (e.g. fluorescent proteins, STOP cassettes) usually requires the design of the targeting strategy, production of Cas9 mRNA and 2 or more gRNA, preparation of donor dsDNA templates, microinjection of one-cell embryos, and screening of founder mice.

Please see fees for pricing information.