CRISPR/Cas9 MOUSE


TARGETING CORE

Henao-Mejia Lab




Services

DNA

KO Mice

Generation of knock-out (KO) mice usually requires the design of the targeting strategy, production of Cas9 mRNA and 2 or more gRNAs, microinjection of one-cell embryos, and screening of founder mice. Double, triple or quadruple KO mice of different genes can be accomplished with one injection.

Please see fees for each of these processes.
DNA

KI Mice - Small Sequences

Generation of knock-in (KI) mice containing small sequences (e.g. point mutations, tags) usually requires the design of the targeting strategy, production of Cas9 mRNA and a gRNA, preparation of donor ssDNA templates, microinjection of one-cell embryos, and screening of founder mice.

Please see fees for each of these processes.
DNA

cKO Mice

Generation of conditional knock-out (cKO) mice usually requires the design of the targeting strategy, production of Cas9 mRNA and 2 gRNAs, preparation of 2 donor ssDNA templates containing loxp sites, microinjection of one-cell embryos, and screening of founder mice.

Please see fees for each of these processes.
DNA

KI Mice - Large Sequences

Generation of knock-in (KI) mice containing large sequences (e.g. fluorescent proteins, STOP cassettes) usually requires the design of the targeting strategy, production of Cas9 mRNA and 2 or more gRNA, preparation of donor dsDNA templates, microinjection of one-cell embryos, and screening of founder mice.

Please see fees for more information

about us

Mission Statement

To streamline procedures that facilitate investigators' use of the CRISPR/Cas9 genome editing technology for the rapid and economic generation of novel mouse genetic tools.

For further information, please contact Dr. Jorge Henao-Mejia or Dr. Jean Richa


Contact Us

BRB II/III
421 Curie Blvd
University of Pennsylvania
Philadelphia, PA 19104

Jorge Henao-Mejia M.D., Ph.D.
jhena@mail.med.upenn.edu
215-898-2212